Natural/synthetic drug screening

Natural/synthetic drug screening

Natural products have been the main members of cancer chemotherapy for the at least last decades. This laboratory is concerned about all experimental works associated to the broadcast and elucidation of novel native compounds extracted from nature and spotlights on the medicinal plants with antioxidant, antimicrobial and anti-cancer properties. Another goal of the Nanobiomaterial lab is assessment of pharmaceutical properties of pure extracted compounds through different cellular and molecular pathways. The laboratory is also fill the gaps between traditional medicine and high-tech sciences through formulation of natural pure products in different pharmaceutical tagged vehicles such as Solid Lipid Nanoparticles, Magnetic Nanoparticle, Nanostructured Lipid Carrier and so on for targeted drug delivery. In this lab, the biotechnological works, which improve the quality and quantity of biomaterials, is also set up.

Technical approach

Extraction of natural products

Natural product extraction using dry and wet methods
Essential oil extraction using Clevenger apparatus
Evaporator rotary

Purification of natural products

Analytical and preparative- Thin Layer Chromatography (TLC)
Vacuum Liquid Chromatography (VLC)
Column Chromatography (CC)
Semi-Preparative High Pressure Liquid Chromatography (Semi-Prep HPLC)

Identification of natural products

UV-Visible Spectrometry
H and C NMR (in collaboration with other research centers)

Improving the quality and quantity of biomaterials

Shaker Incubator
Class I and II box hoods

Lipidic Nanoparticle synthesizing

Ultra-homogenizer
Dialysis

Assessment of natural products properties

Anti-oxidant activity assay (DPPH)
Anti-microbial activity assays (In collaboration with Molecular Medicine & Therapy Lab)
Anti-angiogenesis activity assay (Chorioallantoic Membrane Angiogenesis Assay

Research Leader

Professor. Hossein Nazemiyeh, (PharmD; PhD)
Dr. Morteza Eskandani (M.Sc, Ph.D)

Research Assistants

Dr. Faezeh Bakhshi (Pharm.D.)

Post-doc researchers
Will be updated ASAP.

Students

PhD students
Will be updated ASAP.

Selected Publication based on scopus
Last two years publications (2017-2018)

1. Abbasian M, Roudi MM, Mahmoodzadeh F, Eskandani M, Jaymand M. Chitosan-grafted-poly(methacrylic acid)/graphene oxide nanocomposite as a pH-responsive de novo cancer chemotherapy nanosystem. International Journal of Biological Macromolecules 2018; 118: 1871-9. doi:10.1016/j.ijbiomac.2018.07.036. https://www.scopus.com/inward/record.uri?eid=2-s2.0-85050084919&doi=10.1016%2fj.ijbiomac.2018.07.036&partnerID=40&md5=33428681c4201bab9082c7bb941cc54b

2. Ahani N, Sangtarash MH, Houshmand M, Eskandani MA. Genipin induces cell death via intrinsic apoptosis pathways in human glioblastoma cells. Journal of Cellular Biochemistry 2018. doi:10.1002/jcb.27512. https://www.scopus.com/inward/record.uri?eid=2-s2.0-85052794062&doi=10.1002%2fjcb.27512&partnerID=40&md5=410af271da807892a0ed764b02e6e5d0

3. Ahmadi R, Eskandani MA, Saadati D. Evaluation of antimicrobial effect of Iranian sumac on Bacillus cereus in a commercial barley soup. Slovenian Veterinary Research 2017; 54: 65-9. https://www.scopus.com/inward/record.uri?eid=2-s2.0-85021950210&partnerID=40&md5=95ed0184d45c16ac129fc23c94737677

4. Alizadeh N, Eskandani M, Tondro K, Rashidi MR, Nazemiyeh H. Inhibitory effects of flavonolignans from silybum marianum (L.) gaertn (milk thistle) on function of aldehyde oxidase and xanthine oxidase in rats. Letters in Drug Design and Discovery 2018; 15: 256-62. doi:10.2174/1570180814666170420110005. https://www.scopus.com/inward/record.uri?eid=2-s2.0-85042142636&doi=10.2174%2f1570180814666170420110005&partnerID=40&md5=9d7d71936d1119d1160c37f0e2f402ea

5. Anvari D, Saadati D, Nabavi R, Alipour Eskandani M. Epidemiology and molecular prevalence of Toxoplasma gondii in cattle Slaughtered in Zahedan and Zabol Districts, South East of Iran. Iranian Journal of Parasitology 2018; 13: 114-9. https://www.scopus.com/inward/record.uri?eid=2-s2.0-85044081224&partnerID=40&md5=92e5d9883fc9d43ae8b4ca4b0333f6e2

6. Asgharzadeh MR, Barar J, Pourseif MM, Eskandani M, Niya MJ, Mashayekhi MR, et al. Molecular machineries of pH dysregulation in tumor microenvironment: Potential targets for cancer therapy. BioImpacts 2017; 7: 115-33. doi:10.15171/bi.2017.15. https://www.scopus.com/inward/record.uri?eid=2-s2.0-85027460324&doi=10.15171%2fbi.2017.15&partnerID=40&md5=0829735dfa89423eb093a6fc07cea2ec

7. Bahadori MB, Eskandani M, De Mieri M, Hamburger M, Nazemiyeh H. Anti-proliferative activity-guided isolation of clerodermic acid from Salvia nemorosa L.: Geno/cytotoxicity and hypoxia-mediated mechanism of action. Food and Chemical Toxicology 2018; 120: 155-63. doi:10.1016/j.fct.2018.06.060. https://www.scopus.com/inward/record.uri?eid=2-s2.0-85049924390&doi=10.1016%2fj.fct.2018.06.060&partnerID=40&md5=173289da0f078ba950820cc051e3b2a1

8. Eskandani M, Vandghanooni S, Barar J, Nazemiyeh H, Omidi Y. Cell physiology regulation by hypoxia inducible factor-1: Targeting oxygen-related nanomachineries of hypoxic cells. International Journal of Biological Macromolecules 2017; 99: 46-62. doi:10.1016/j.ijbiomac.2016.10.113. https://www.scopus.com/inward/record.uri?eid=2-s2.0-85013761924&doi=10.1016%2fj.ijbiomac.2016.10.113&partnerID=40&md5=4d609bc880884abb22a518041b6a9202

9. Hamishehkar H, Bahadori MB, Vandghanooni S, Eskandani M, Nakhlband A, Eskandani M. Preparation, characterization and anti-proliferative effects of sclareol-loaded solid lipid nanoparticles on A549 human lung epithelial cancer cells. Journal of Drug Delivery Science and Technology 2018; 45: 272-80. doi:10.1016/j.jddst.2018.02.017. https://www.scopus.com/inward/record.uri?eid=2-s2.0-85044114825&doi=10.1016%2fj.jddst.2018.02.017&partnerID=40&md5=cb4a29ff0068898cf3beef801b1e5554

10. Jafarizad A, Taghizadehgh-Alehjougi A, Eskandani M, Hatamzadeh M, Abbasian M, Mohammad-Rezaei R, et al. PEGylated graphene oxide/Fe<inf>3</inf>O<inf>4</inf>site: Synthesis, characterization, and evaluation of its performance as de novo drug delivery nanosystem. Bio-Medical Materials and Engineering 2018; 29: 177-90. doi:10.3233/BME-171721. https://www.scopus.com/inward/record.uri?eid=2-s2.0-85043572640&doi=10.3233%2fBME-171721&partnerID=40&md5=604dbe0eba079c4e81465ea27928fcea

11. Nakhlband A, Eskandani M, Omidi Y, Saeedi N, Ghaffari S, Barar J, et al. Combating atherosclerosis with targeted nanomedicines: Recent advances and future prospective. BioImpacts 2018; 8: 59-75. doi:10.15171/bi.2018.08. https://www.scopus.com/inward/record.uri?eid=2-s2.0-85044298919&doi=10.15171%2fbi.2018.08&partnerID=40&md5=c44d352eea40fe79a5840b756e3d8c49

12. Nakhlband A, Eskandani M, Saeedi N, Ghafari S, Omidi Y, Barar J, et al. Marrubiin-loaded solid lipid nanoparticles’ impact on TNF-α treated umbilical vein endothelial cells: A study for cardioprotective effect. Colloids and Surfaces B: Biointerfaces 2018; 164: 299-307. doi:10.1016/j.colsurfb.2018.01.046. https://www.scopus.com/inward/record.uri?eid=2-s2.0-85041484199&doi=10.1016%2fj.colsurfb.2018.01.046&partnerID=40&md5=75a93e53a55737af8ff5751d49c96e8d

13. Nazemi K, Salari S, Eskandani MA. Assessment of the escherichia coli pollution in drinking water and water sources in sistan, iran. Journal of Water Reuse and Desalination 2018; 8: 386-92. doi:10.2166/wrd.2017.146. https://www.scopus.com/inward/record.uri?eid=2-s2.0-85047217532&doi=10.2166%2fwrd.2017.146&partnerID=40&md5=ec1779bc5da8845e7ab73a5b0bfe6a6d

14. Rahmanian N, Eskandani M, Barar J, Omidi Y. Recent trends in targeted therapy of cancer using graphene oxide-modified multifunctional nanomedicines. Journal of Drug Targeting 2017; 25: 202-15. doi:10.1080/1061186X.2016.1238475. https://www.scopus.com/inward/record.uri?eid=2-s2.0-85013790692&doi=10.1080%2f1061186X.2016.1238475&partnerID=40&md5=bd598c4cfefb872b3e2a2475014d55e9

15. Ranjbar-Navazi Z, Eskandani M, Johari-Ahar M, Nemati A, Akbari H, Davaran S, et al. Doxorubicin-conjugated D-glucosamine- and folate- bi-functionalised InP/ZnS quantum dots for cancer cells imaging and therapy. Journal of Drug Targeting 2018; 26: 267-77. doi:10.1080/1061186X.2017.1365876. https://www.scopus.com/inward/record.uri?eid=2-s2.0-85028533760&doi=10.1080%2f1061186X.2017.1365876&partnerID=40&md5=ecb973a8ad3ac8a67a759bfe5c6554a7

16. Salmanzadeh R, Eskandani M, Mokhtarzadeh A, Vandghanooni S, Ilghami R, Maleki H, et al. Propyl gallate (PG) and tert-butylhydroquinone (TBHQ) may alter the potential anti-cancer behavior of probiotics. Food Bioscience 2018; 24: 37-45. doi:10.1016/j.fbio.2018.05.005. https://www.scopus.com/inward/record.uri?eid=2-s2.0-85047079935&doi=10.1016%2fj.fbio.2018.05.005&partnerID=40&md5=86ec63b293c96c30b9a73dbb9b725882

17. Soleymani J, Hasanzadeh M, Eskandani M, Khoubnasabjafari M, Shadjou N, Jouyban A. Electrochemical sensing of doxorubicin in unprocessed whole blood, cell lysate, and human plasma samples using thin film of poly-arginine modified glassy carbon electrode. Materials Science and Engineering C 2017; 77: 790-802. doi:10.1016/j.msec.2017.03.257. https://www.scopus.com/inward/record.uri?eid=2-s2.0-85017176609&doi=10.1016%2fj.msec.2017.03.257&partnerID=40&md5=2cc028e231470e8bc56f40af9a154c01

18. Vandghanooni S, Eskandani M, Barar J, Omidi Y. Bispecific therapeutic aptamers for targeted therapy of cancer: a review on cellular perspective. Journal of Molecular Medicine 2018; 96: 885-902. doi:10.1007/s00109-018-1669-y. https://www.scopus.com/inward/record.uri?eid=2-s2.0-85050732999&doi=10.1007%2fs00109-018-1669-y&partnerID=40&md5=74c22af238864e7d1d869770b489bc80

19. Vandghanooni S, Eskandani M, Barar J, Omidi Y. Recent advances in aptamer-armed multimodal theranostic nanosystems for imaging and targeted therapy of cancer. European Journal of Pharmaceutical Sciences 2018; 117: 301-12. doi:10.1016/j.ejps.2018.02.027. https://www.scopus.com/inward/record.uri?eid=2-s2.0-85043258625&doi=10.1016%2fj.ejps.2018.02.027&partnerID=40&md5=dd05d32378ddc2b49636836862d6604b

20. Zamanlu M, Eskandani M, Mohammadian R, Entekhabi N, Rafi M, Farhoudi M. Spectrophotometric analysis of thrombolytic activity: SATA assay. BioImpacts 2018; 8: 31-8. doi:10.15171/bi.2018.05. https://www.scopus.com/inward/record.uri?eid=2-s2.0-85044265885&doi=10.15171%2fbi.2018.05&partnerID=40&md5=2d24d7004554008421046a67dbbc318a

21. Zamanlu M, Farhoudi M, Eskandani M, Mahmoudi J, Barar J, Rafi M, et al. Recent advances in targeted delivery of tissue plasminogen activator for enhanced thrombolysis in ischaemic stroke. Journal of Drug Targeting 2018; 26: 95-109. doi:10.1080/1061186X.2017.1365874. https://www.scopus.com/inward/record.uri?eid=2-s2.0-85041324095&doi=10.1080%2f1061186X.2017.1365874&partnerID=40&md5=89cb1d43d0465373462ee13f3fda5150

22. Afshar FH, Delazar A, Nazemiyeh H, Nahar L, Moghaddam SB, Mbaebie BO, et al. Melilotoside derivatives from Artemisia splendens (Asteraceae). Records of Natural Products 2017; 11: 43-50. https://www.scopus.com/inward/record.uri?eid=2-s2.0-84988961453&partnerID=40&md5=e32a424b7164d2ec00603ca8cc41f9d9

23. Delazar A, Nazemiyeh H, Afshar F, Barghi N, Esnaashari S, Asgharian P. Chemical compositions and biological activities of Scutellaria pinnatifida A. Hamilt aerial parts. Research in Pharmaceutical Sciences 2017; 12: 187-95. doi:10.4103/1735-5362.207199. https://www.scopus.com/inward/record.uri?eid=2-s2.0-85020242664&doi=10.4103%2f1735-5362.207199&partnerID=40&md5=6ae6b1e8918067c6bc89bca4d502a89f

24. Khodadadi A, Nemati M, Tamizi E, Nazemiyeh H. Facile and accelerated method for detection of adulteration in commercially available lime juice products in Iranian market. Pharmaceutical Sciences 2018; 24: 148-56. doi:10.15171/PS.2018.22. https://www.scopus.com/inward/record.uri?eid=2-s2.0-85049353569&doi=10.15171%2fPS.2018.22&partnerID=40&md5=a5e1190e47c0213efc8c91eafdab9ecb

25. Kirimer N, Suleymanov TA, Kurkcuoglu M, Nazemiyeh H, Shukurova AS, Jouyban A. Essential oil constituents of phlomis pungens willd. From Azerbaijan. Journal of Essential Oil-Bearing Plants 2017; 20: 1492-501. doi:10.1080/0972060X.2017.1407261. https://www.scopus.com/inward/record.uri?eid=2-s2.0-85046846179&doi=10.1080%2f0972060X.2017.1407261&partnerID=40&md5=54a71076905355763f47631293cfd783

26. Mahmoodpoor A, Medghalchi M, Nazemiyeh H, Asgharian P, Shadvar K, Hamishehkar H. Effect of Cucurbita Maxima on control of blood glucose in diabetic critically Ill patients. Advanced Pharmaceutical Bulletin 2018; 8: 347-51. doi:10.15171/apb.2018.040. https://www.scopus.com/inward/record.uri?eid=2-s2.0-85048896288&doi=10.15171%2fapb.2018.040&partnerID=40&md5=c83638e94a6379991efaac070a7d0422

27. Moini Jazani A, Hamdi K, Tansaz M, Nazemiyeh H, Sadeghi Bazargani H, Fazljou SMB, et al. Herbal Medicine for Oligomenorrhea and Amenorrhea: A Systematic Review of Ancient and Conventional Medicine. BioMed research international 2018; 2018: 3052768. doi:10.1155/2018/3052768. https://www.scopus.com/inward/record.uri?eid=2-s2.0-85053558827&doi=10.1155%2f2018%2f3052768&partnerID=40&md5=51d3d6332cf02ae450e08a248104a598

28. Movahhedin N, Nazemiyeh H, Barar J, Esnaashari S, Movahhedin AH. Chemical constituent and biological activities of spatoglossum asperum J. Agardh from Oman sea. Letters in Drug Design and Discovery 2018; 15: 263-9. doi:10.2174/1570180814666170407103936. https://www.scopus.com/inward/record.uri?eid=2-s2.0-85042144419&doi=10.2174%2f1570180814666170407103936&partnerID=40&md5=f1facf3f7310b892fdf34723e9fa0749

29. Nasimi Doost Azgomi R, Nazemiyeh H, Sadeghi Bazargani H, Fazljou SMB, Nejatbakhsh F, Moini Jazani A, et al. Comparative evaluation of the effects of Withania somnifera with pentoxifylline on the sperm parameters in idiopathic male infertility: A triple-blind randomised clinical trial. Andrologia 2018; 50. doi:10.1111/and.13041. https://www.scopus.com/inward/record.uri?eid=2-s2.0-85050992232&doi=10.1111%2fand.13041&partnerID=40&md5=73724f1ca6d94d070b64f0c4831e0e62

30. Yaripour S, Delnavazi MR, Asgharian P, Valiyari S, Tavakoli S, Nazemiyeh H. A survey on phytochemical composition and biological activity of Zygophyllum Fabago from Iran. Advanced Pharmaceutical Bulletin 2017; 7: 109-14. doi:10.15171/apb.2017.014. https://www.scopus.com/inward/record.uri?eid=2-s2.0-85017571294&doi=10.15171%2fapb.2017.014&partnerID=40&md5=d17d2431b50bc1d4f154a4ee13ddd0d8